GeneRuler Ultra Low Range DNA Ladder, Ready-to-Use 10 to 300 bp

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The ladder is recommended for analysis of small dsDNA fragments on agarose or polyacrylamide gels. Ideal for siRNA analysis. Premixed with Loading Dye for direct loading onto gel.
  
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GeneRuler Ultra Low Range DNA Ladder - 300, 200, 150, 100, 75, 50, 35, 25, 20, 15 and 10 bp bands.

Thermo Scientific GeneRuler Ultra Low Range DNA Ladder, ready-to-use is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Ultra Low Range DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading on gel.

The ladder is recommended for analysis of siRNA (see Figure 1 in Supporting Data).

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification
  • Ideal for non-denaturing polyacrylamide gel electrophoresis
  • Ideal for analysis of siRNA
  • Sharp bands
  • Bright reference bands (given in red)
  • Can be directly loaded and are stable at room temperature for 6 months
  • Supplied with loading dye for sample DNA
  
6× DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Bands300, 200, 150, 100, 75, 50, 35, 25, 20, 15, and 10 bp bands.
GeneRuler Storage and Loading Buffer (for ready-to-use ladders)10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF, and 10% glycerol.
HazardousNo
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage Condition-20 C
siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

Figure 1 | siRNA gel analysis by GeneRuler and O'GeneRuler Ultra Low Range DNA ladders
Electrophoresis conditions: 5% agarose gel, 1X TBE buffer, 7 V/cm, 45 min.
1 – GeneRuler Ultra Low Range DNA Ladder
2 – 21 bp siRNA (0.5 µg), premixed with 6X DNA Loading Dye


References

Citations