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Lambda DNA/HindIII Marker, Ready-to-Use

Store at room temperature

A conventional ready-to-use marker composed of 8 sharp bands designed for sizing and approximate quantification of large double-stranded DNA fragments in agarose gels.
  
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Lambda DNA/HindIII Marker, 2 

The Thermo Scientific Lambda DNA/HindIII Marker, ready-to-use is recommended for sizing of linear double-stranded large DNA on agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/HindIII Marker, ready-to-use is premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose gels.

Highlights

  • Sizing and approximate quantification of large DNA fragments
  • Sharp bands
  • Premixed with 6X DNA Loading Dye for a direct loading (after heating) and for room temperature storage
  • Supplied with loading dye for sample DNA

Note

The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes.

  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer (for ready-to-use markers)10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF, and 10% glycerol.
Storage NotesStore at room temperature or at 4°C for up to 6 monthsFor longer periods, store at -20°C.