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MassRuler Express Forward and Reverse DNA Ladders Mixes

Ready-to-use, 100 to 10,000 bp

DNA Ladder mixes specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions.


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Description
Specifications
Supporting Data
References
Resources

Thermo Scientific MassRuler Express Forward and Reverse DNA Ladder mixes are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions (see Table 1). In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size whereas, in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights

Accurate DNA sizing and quantification – DNA ladder fragments are quantified spectrophotometrically
Fast and reliable DNA quantification – short separation distances under various electrophoresis conditions
Sharp bands
Easy-to-remember fragment sizes and quantities
Ready-to-use – premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage
Supplied with loading dye for sample DNA

MassRuler Express Forward and Reverse DNA Ladders Mixes, ready-to-use


Storage Condition	-20 C
Hazardous	No
6X MassRuler DNA Loading Dye	10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol, and 60 mM EDTA.
Quality Control	Well-defined bands are formed during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage	Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer	10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.