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MassRuler Express LR Forward and Reverse DNA Ladders

Ready-to-use, 100 to 1000 bp

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DNA ladders specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions.
  
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Thermo Scientific MassRuler Express LR Forward and Reverse DNA ladders are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions. In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size, whereas in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights

  • Accurate DNA sizing and quantification – DNA ladder fragments are quantified spectrophotometrically
  • Fast and reliable DNA quantification – short separation distances under various electrophoresis conditions
  • Sharp bands
  • Easy-to-remember fragment sizes and quantities
  • Ready-to-use – premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage
  • Supplied with loading dye for sample DNA
MassRuler Express LR Forward and Reverse DNA Ladders, ready-to-use 
  
Storage Condition-20 C
HazardousNo
6X MassRuler DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol, and 60 mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
MassRuler Express LR DNA Ladder Separation Guide for Various Electrophoresis Conditions

Table 1. MassRuler Express LR DNA Ladder Separation Guide for Various Electrophoresis Conditions

MassRuler Express LR DNA Ladder Separation Guide for Various Electrophoresis Conditions