Home | Molecular Biology | Electrophoresis & Nucleic Acid Purification | Nucleic Acid Electrophoresis | DNA Electrophoresis | Custom DNA Ladders

NoLimits 10 bp DNA Fragment

Store at -20°C

NoLimits is a collection of 36 chromatography-purified individual DNA fragments ranging from 10-20,000 bp for applications where a custom DNA size standard is required.
  
Loading...

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.

Plasmid DNA is digested with Thermo Scientific Restriction Enzymes, and the individual DNA fragments are chromatography-purified from the digestion mixture. As a result, we offer exceptionally pure DNA fragments that are free of any truncated or degraded molecules. Our custom DNA fragments are the best choice for applications, ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Highlights

  • Broad range: 10-20,000 bp
  • Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
  • Precise DNA concentration 
  • Chromatography-purified individual DNA fragments

Applications

  • Gel electrophoresis
  • Capillary electrophoresis
  • DNA quantification
  • HPLC analysis
  • Applications where a custom DNA size standard is required.
  
Storage Condition-20 C
HazardousNo
Quality Control
  • Tested on an Agilent 2100 bioanalyzer™ and by appropriate gel electrophoresis.
  • DNA concentration determined spectrophotometrically.
  • The absence of nucleases confirmed by appropriate tests.
Storage Buffer (TE buffer)10 mM Tris-HCl (pH 7.6) with 1 mM EDTA.
NoLimits DNA fragments from 300 bp to 10 kb

NoLimits DNA fragments from 300 bp to 10 kb

NoLimits DNA fragments from 300 bp to 10 kb

1.1% TopVision LE GQ Agarose,
1X TAE, 7 V/cm, 30 min.
NoLimits DNA fragments from 300 bp to 10 kb