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O’GeneRuler Plus DNA Ladder, Ready-to-Use, 100-3000 bp

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100 bp DNA ladder for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is premixed with blue and yellow tracking dyes.
  
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GeneRuler 100 bp Plus DNA Ladder 

Thermo Scientific O'GeneRuler 100 bp Plus DNA Ladder, ready-to-use is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

O'GeneRuler 100 bp Plus DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel, orange G dye migrates at 50 bp.

The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Can be directly loaded and are stable at room temperature for 6 months 
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X Orange DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
O'GeneRuler™ Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, and 10% glycerol.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.