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O’GeneRuler DNA Ladder, Ready-to-Use 50-1000 bp

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50 bp DNA ladder for sizing and approximate quantification of double-stranded DNA on agarose gels. The ladder is premixed with blue and yellow tracking dyes.
  
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GeneRuler 50 bp DNA Ladder 

Thermo Scientific O'GeneRuler 50 bp DNA Ladder, ready-to-use is recommended for sizing and approximate quantification of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

O'GeneRuler 50 bp DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel, orange G dye migrates at 50 bp. Therefore, O'GeneRuler 50 bp DNA Ladder is ideal when visualization of small DNA fragments is important.

The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Can be directly loaded and are stable at room temperature for 6 months 
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X Orange DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
O'GeneRuler Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, and 10% glycerol.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.