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Thermo Scientific O'GeneRuler Express DNA Ladder 100-5000 bp, ready-to-use is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
O'GeneRuler Express DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel, orange G dye migrates at 50 bp.
The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.
Fast separation of GeneRuler Express DNA Ladder at high voltage (23 V/cm).
Electrophoresis conditions: 0.5 µg/lane, 1% TopVision LE GQ Agarose, 1X TAE, 23 V/cm, 5- to 5 minutes