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O’GeneRuler Express DNA Ladder, Ready-to-Use 100-5000 bp

DNA ladder for fast sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is premixed with blue and yellow tracking dyes.


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Description
Specifications
Supporting Data
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Thermo Scientific O'GeneRuler Express DNA Ladder 100-5000 bp, ready-to-use is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.

O'GeneRuler Express DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel, orange G dye migrates at 50 bp.

The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

Ideal for both DNA sizing and approximate quantification
Ideal for fast separation under a wide range of electrophoresis conditions
Sharp bands
Bright reference bands (given in red)
Can be directly loaded and are stable at room temperature for 6 months
Supplied with loading dye for sample DNA


Storage Condition	-20 C
Hazardous	No
6X Orange DNA Loading Dye	10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
O'GeneRuler™ Storage and Loading Buffer	10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, and 10% glycerol.
Quality Control	Well-defined bands are formed in 5 to 10 minutes during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage	Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.