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O’GeneRuler Ultra Low Range DNA Ladder, Ready-to-Use 10-300 bp

Low range DNA ladder for sizing and approximate quantification of double-stranded DNA on agarose gels. The ladder is premixed with blue and yellow tracking dyes.


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Description
Specifications
Supporting Data
References
Resources

Thermo Scientific O'GeneRuler Ultra Low Range DNA Ladder 10-300 bp, ready-to-use is recommended for sizing and approximate quantification of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.

O'GeneRuler Ultra Low Range DNA Ladder, ready-to-use is premixed with 6X Orange DNA Loading Dye (containing xylene cyanol FF and orange G) for direct loading on gel. In a 1% agarose gel, orange G dye migrates at 50 bp. O'GeneRuler Ultra Low Range DNA Ladder is ideal when visualization of small DNA fragments is important.

The ladder is recommended for analysis of siRNA (see Figure 1) and is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

Ideal for both DNA sizing and approximate quantification
Ideal for non-denaturing polyacrylamide gel electrophoresis
Ideal for analysis of siRNA
Sharp bands
Bright reference bands (given in red)
Can be directly loaded and are stable at room temperature for 6 months
Supplied with loading dye for sample DNA


Storage Condition	-20 C
Hazardous	No
6X Orange DNA Loading Dye	10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
O'GeneRuler Storage and Loading Buffer	10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, and 10% glycerol.
Quality Control	Well-defined bands are formed during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage	Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.

siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

siRNA gel analysis by GeneRuler and O'GeneRuler Ultra Low Range DNA ladders.

Electrophoresis conditions: 5% agarose gel, 1X TBE buffer, 7 V/cm, 45 minutes.
1 – GeneRuler Ultra Low Range DNA Ladder
2 – 21 bp siRNA (0.5 µg), premixed with 6X DNA Loading Dye