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O’RangeRuler 20 bp DNA Ladder, Ready-to-Use 20-300 bp


DNA step ladder of 20 bp increments for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels.
  
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O'RangeRuler 20 bp DNA Ladder, ready-to-use 

Thermo Scientific O'RangeRuler 20 bp DNA Ladder 20-300 bp is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is a step ladder with 20 bp differences between the DNA fragments. It consists of purified and ligated blunt-ended basic unit repeats of 20 bp. Not recommended for DNA quantification.

O'RangeRuler 20 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA. Orange G dye can be used to monitor DNA migration in agarose or polyacrylamide gels and migrates at 50 bp in a 1% agarose gel. It is the preferable dye when visualization of small DNA fragments is important.

Highlights

  • Step ladder with 20 bp increments 
  • Sharp bands, easy-to-remember fragment sizes 
  • Evenly spaced reference bands (given in red)
  • Ready-to-use – premixed with 6X Orange Loading Dye for direct loading and room temperature storage
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X Orange DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.