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O’RangeRuler 50 bp DNA Ladder, Ready-to-Use 50-1000 bp


DNA step ladder of 50 bp increments for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels.
  
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O'RangeRuler 50 bp DNA Ladder, ready-to-use 

Thermo Scientific O'RangeRuler 50 bp DNA Ladder 50-1000 bp is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is a step ladder with 50 bp differences between the DNA fragments. It consists of purified and ligated blunt ended basic unit repeats of 50 bp. Not recommended for DNA quantification.

O'RangeRuler 50 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA. Orange G dye can be used to monitor DNA migration in agarose or polyacrylamide gels and migrates at 50 bp in a 1% agarose gel. It is the preferable dye when visualization of small DNA fragments is important.

Highlights

  • Step ladder with 50 bp increments 
  • Sharp bands, easy-to-remember fragment sizes 
  • Evenly spaced reference bands (given in red)
  • Ready-to-use – premixed with 6X Orange Loading Dye for direct loading and room temperature storage
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X Orange DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.