Previously added items:
0.5µg/lane, 8cm length gel, 1X TBE, 5V/cm, 1.5h. Bands visible: 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34 and 34 bp.
Thermo Scientific pUC19 DNA/MspI (HpaII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pUC19 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
pUC19 DNA/MspI (HpaII) Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels.
Bands indicated ** form an anomalous pattern on polyacrylamide gels. The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of the respective DNA sequences.