RiboRuler High Range RNA Ladder Ready-to-Use, 200 to 6000 bases

Available On-Site


Ready-to-use RNA Ladder composed of 8 chromatography purified single-stranded RNA transcripts for RNA sizing and quantification on native or denaturing gels.
  
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4 µL of ladder per lane, 8 cm gel length, 1X TAE, 5 V/cm. Bands visible at 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200 bp.

RiboRuler High Range RNA Ladder 200 to 6000 bases, ready-to-use is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments. Ladder is premixed with loading dye.

Thermo Scientific RiboRuler High Range RNA Ladder, ready-to-use, is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

Highlights

  • Sharp bands of uniform intensity
  • Easy-to-remember band sizes and quantities
  • Predetermined concentration of ladder bands allows for approximate RNA quantification
  • Supplied with 2X RNA Loading Dye
  • Stable for 6 months at -20°C

Applications

  • RNA sizing and quantification on native or denaturing gels
  • Northern blotting
  
2X RNA Loading Dye95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, and 0.5 mM EDTA.
HazardousNo
Quality Control
  • Tested in gel electrophoresis.
  • RNA concentration is determined spectrophotometrically.
  • The absence of ribonucleases confirmed by appropriate tests.
StorageStore at -20°C for 6 months or at -70°C for longer periods.
Storage and RNA Loading Buffer (for ready-to-use ladders)47.5% formamide, 0.0125% SDS, 0.0125% bromophenol blue, 0.0125% xylene cyanol FF, 0.0125% ethidium bromide, and 0.75 mM EDTA.
Storage Condition-20 C

References

Citations