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RiboRuler High Range RNA Ladder 200 to 6000 bases

Store at -20°C

RNA Ladder is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long) for RNA sizing and quantification on native or denaturing gels.
  
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2 µL of ladder per lane, 8cm gel length, 1X TAE, 5 V/cm. Bands visible at 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200bp.

Thermo Scientific RiboRuler High Range RNA Ladder 200 to 6000 bases is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

The RiboRuler High Range RNA Ladder is available in 1 mM EDTA, pH 6.0.

RiboRuler High Range RNA Ladder is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

RiboRuler High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.

Highlights

  • Sharp bands of uniform intensity
  • Easy-to-remember band sizes and quantities
  • Predetermined concentration of ladder bands allows for approximate RNA quantification
  • Supplied with 2X RNA Loading Dye
  • Stable for 6 months at -20°C

Applications

  • RNA sizing and quantification on native or denaturing gels
  • Northern blotting
  
Storage Condition-20 C
HazardousNo
2X RNA Loading Dye95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide and 0.5 mM EDTA.
Quality Control
  • Tested in gel electrophoresis.
  • RNA concentration is determined spectrophotometrically.
  • The absence of ribonucleases confirmed by appropriate tests.
StorageStore at -20°C for 6 months or at -70°C for longer periods.
Storage Buffer1 mM EDTA (pH 6.0).