Home | Molecular Biology | Electrophoresis & Nucleic Acid Purification | Nucleic Acid Electrophoresis | RNA Electrophoresis | RNA Ladders

RiboRuler Low Range RNA Ladder 100-1000 bp


RiboRuler Low Range RNA Ladder is a mixture of 7 chromatography purified single-stranded RNA transcripts (100 to 1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
  
Loading...
4 µL of ladder per lane, 8cm gel length, 1X TAE, 5 V/cm. Bands visible at 1000, 800, 600, 400, 300, 200, and 100bp. 

Thermo Scientific RiboRuler Low Range RNA Ladder 100-1000 bp is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

Ladder is available in 1 mM EDTA, pH 6.0.

RiboRuler Low Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see Photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.

RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see Protocol) and is ideal for Northern blots.

Highlights

  • Sharp bands of uniform intensity 
  • Easy-to-remember band sizes and quantities
  • Approximate RNA quantification by predetermined concentration of ladder bands
  • Supplied with 2X RNA Loading Dye 
  • Stable for 6 months at -20°C 

Applications

  • RNA sizing and quantification on native or denaturing gels
  • Northern blotting.
  
Storage Condition-20 C
HazardousNo
2X RNA Loading Dye95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, and 0.5 mM EDTA.
Quality Control
  • Tested in gel electrophoresis.
  • RNA concentration is determined spectrophotometrically.
  • The absence of ribonucleases confirmed by appropriate tests.
StorageStore at -20°C for 6 months or at -70°C for longer periods.
Storage Buffer1 mM EDTA (pH 6.0).