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TopVision Agarose

Store at room temperature

TopVision Agarose is highly purified agarose suitable for analytical and preparative electrophoresis of nucleic acids.
  
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Separation of DNA ladders in TopVision LE GQ Agarose

Separation of Thermo Scientific RiboRuler RNA Ladders in TopVision LE GQ Agarose. Electrophoresis conditions: 1X TAE buffer, 7 V/cm, 40 min.

1 – RiboRuler Low Range RNA Ladder, 2 µL/lane, 2% gel
2 – RiboRuler High Range RNA Ladder, 2 µL/lane, 1% gel

 

Thermo Scientific TopVision Agarose provides optimal concentration between 0.7 to 2% in all typical buffer systems. It is a highly purified agarose with very low EEO values certified by strict quality control test procedures.

Highlights

  • Optimal concentration between 0.4 to 5% in all typical buffer systems
  • GQ (Genetic Quality) certified, which ensures that nucleic acids recovered from preparative gels can be used for downstream applications (enzymatic reactions, etc.)
  • Low DNA/RNA binding
  • Excellent gel transparency
  • DNase and RNase free
  • Suitable for RNA analysis

Applications

  • Analytical electrophoresis of nucleic acids
  • Preparative electrophoresis
  • Blotting assays
  
Storage ConditionAmbient
HazardousNo
Electroendosmosis EEO0.05-0.13
Gel strength (1% gel)> 1200 g/cm2 
Gel strength (1.5% gel)> 2500 g/cm2 
Gelling temperature36 ± 1.5°C
Melting temperature88 ± 1.5°C
Moisture< 7%
Quality ControlThe absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Separation of DNA ladders in TopVision LE GQ Agarose

Separation of DNA ladders in TopVision LE GQ Agarose

Separation of DNA ladders in TopVision LE GQ Agarose

Separation of Thermo Scientific DNA Ladders in TopVision LE GQ Agarose. Electrophoresis conditions: 1% gel, 0.5 µg/lane, 1X TAE, 7 V/cm, 40 min.

1 – GeneRuler DNA Ladder Mix, ready-to-use
2 – GeneRuler 1 kb DNA Ladder, ready-to-use
3 – GeneRuler 1 kb Plus DNA Ladder, ready-to-use
4 – ZipRuler Express DNA Ladder 1, ready-to-use
5 – ZipRuler Express DNA Ladder 2, ready-to-use
6 – GeneRuler Express DNA Ladder, ready-to-use


MSDS & Certificates

pdf   TopVision Agarose - MSDS