Home | Molecular Biology | Molecular Biology Applications | In vitro Transcription | Buffers & Reagents

TopVision Low Melting Point Agarose

Store at room temperature

TopVision Low Melting Point Agarose is used for in-gel enzymatic process experiments and for nucleic acid recovery after electrophoresis.
  
Loading...
Separation of RNA Ladders in TopVision Low Melting Point Agarose

Separation of Thermo Scientific RiboRuler RNA Ladders in TopVision Low Melting Point Agarose.
Electrophoresis conditions: 1X TAE buffer, 7 V/cm, 40 min.

1 – RiboRuler Low Range RNA Ladder, 2 µL/lane, 2% gel
2 – RiboRuler High Range RNA Ladder, 2 µL/lane, 1% gel

Thermo Scientific Top Vision Low Melting Point Agarose is highly purified agarose with a low melting temperature certified by strict quality control test procedures. It is used for in-gel enzymatic process experiments and for nucleic acids recovery from gels using Agarase (#EO0461).

 

Highlights

  • Optimal concentration between 0.7-2% in all typical buffer systems
  • GQ (Genetic Quality) certified, which ensures that nucleic acids recovered from preparative gels can be used for downstream applications (enzymatic reactions etc.)
  • Low DNA/RNA binding 
  • Excellent gel transparency 
  • DNase and RNase free 
  • Suitable for RNA analysis 

Applications

  • Analytical electrophoresis of nucleic acids
  • Preparative electrophoresis
  • In-gel enzymatic processing experiments
  • Preparation of DNA preparative gels in routine molecular biology techniques
  • The DNA recovered from agarose gels after electrophoresis can be used in enzymatic process (restriction, ligation, etc.) (see Reference 1)
  
Storage ConditionAmbient
HazardousNo
Electroendosmosis EEO< 0.12
Gel strength (1% gel)> 250 g/cm2 
Gelling temperature24 to 28°C
Melting temperature< 65.5°C
Moisture< 7%
Quality ControlThe absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Separation of DNA ladders in TopVision Low Melting Point Agarose

Separation of DNA ladders in TopVision Low Melting Point Agarose

Separation of DNA ladders in TopVision Low Melting Point Agarose

Separation of Thermo Scientific DNA Ladders in TopVision Low Melting Point Agarose. Electrophoresis conditions: 1% gel, 0.5 µg/lane, 1X TAE, 7 V/cm, 40 min.

1 – GeneRuler DNA Ladder Mix, ready-to-use
2 – GeneRuler 1 kb DNA Ladder, ready-to-use
3 – GeneRuler 1 kb Plus DNA Ladder, ready-to-use
4 – ZipRuler Express DNA Ladder 1, ready-to-use
5 – ZipRuler Express DNA Ladder 2, ready-to-use
6 – GeneRuler Express DNA Ladder, ready-to-use


References

  1. F. M. Ausubel, Current Protocols in Molecular Biology (John Wiley & Sons, Inc., Brooklyn, New York, 2.6.5-2.6.7, 1999).