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The Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Genomic DNA from various sources purified with the Genomic DNA Purification Kit.Agarose gel (0.7%) electrophoresis analysis of undigested (odd lane numbers) and EcoRI digested (even lane numbers) DNA samples.M – Lambda DNA/HindIII Marker1, 2 – human blood3, 4 – human lymphocytes5, 6 – human placenta7, 8 – mouse liver9, 10 – Hedera helix11, 12 – Haemophilus influenzae13, 14 – E.coli ER226715, 16 – Bacillus subtilis
DNA purification from whole blood