Genomic DNA Purification Kit


Selective detergent-mediated DNA precipitation system for genomic DNA isolation from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
  
Loading...

The Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

  • Efficient – 2 to 10 µg of genomic DNA from 0.2 mL of blood
  • Fast – procedure takes approximately 20 minutes
  • Universal – purifies genomic DNA from various sources
  • Flexible – easy to scale up and down
  • Safe – does not involve phenol extraction

Applications

  • Purification of high-quality genomic DNA from:
    • Whole blood or serum
    • Cell culture
    • Plant tissues
    • Mammalian tissues
    • Epithelium samples
    • Bacteria

Includes

  • Lysis Solution
  • Precipitation Solution
  • NaCl Solution
  • Detailed Protocol
  
HazardousNo
Quality Control
  • The kit is functionally tested for purification of genomic DNA from 0.2 mL of human blood.
  • The typical yield of DNA is 2 to 10 µg.
  • The quality of the purified DNA is evaluated in restriction digestion and in PCR amplification of a single-copy gene.
Storage ConditionAmbient
Genomic DNA purified with the Genomic DNA Purification Kit

Genomic DNA purified with the Genomic DNA Purification Kit

Genomic DNA purified with the Genomic DNA Purification Kit

Genomic DNA from various sources purified with the Genomic DNA Purification Kit.
Agarose gel (0.7%) electrophoresis analysis of undigested (odd lane numbers) and EcoRI digested (even lane numbers) DNA samples.
MLambda DNA/HindIII Marker
1, 2 – human blood
3, 4 – human lymphocytes
5, 6 – human placenta
7, 8 – mouse liver
9, 10Hedera helix
11, 12Haemophilus influenzae
13, 14E.coli ER2267
15, 16Bacillus subtilis


DNA purification from whole blood

DNA purification from whole blood

DNA purification from whole blood

DNA purification from whole blood


References

Citations