GeneJET Gel Extraction Kit

Available On-Site


Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.
  
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Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights

  • Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
  • Fast: procedure takes only 15 minutes
  • Convenient: spin columns are capped and assembled with collection tubes

Applications

  • Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:

Includes

  • Binding Buffer
  • Wash Buffer (concentrated)
  • Elution Buffer (10 mM Tris-HCl, pH 8.5)
  • GeneJET Purification Columns (preassembled with collection tubes)
  • Detailed Protocol
  
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Quality Control
  • The kit is tested in the extraction of 100 bp DNA fragment from a 2% agarose gel and a 5.5 kb DNA fragments from 1% agarose gel according to the protocol.
  • The quality of the extracted DNA is evaluated spectrophotometrically, by agarose gel electrophoresis, digestion with FastDigest restriction enzymes and automated fluorescent sequencing.
Storage ConditionAmbient
Superior extraction of large and small DNA fragments from agarose gels

Superior extraction of large and small DNA fragments from agarose gels

Superior extraction of large and small DNA fragments from agarose gels

Superior extraction of large and small DNA fragments from agarose gels.


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