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The Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Figure 1. | Genomic DNA purified from various sources using the GeneJET Genomic DNA Purification Kit.150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.M – Lambda DNA/HindIII Marker (#SM0101)1 – human bone marrow2 – human blood3 – rat spleen4 – mouse heart5 – mouse liver6 – HeLa cells7 – Jurkat cells8 – E.coli DH5α cells
Figure 2 | Yields of genomic DNA purified using the GeneJET Genomic DNA Purification Kit and kits from other vendors.
Overview of purification procedure.