GeneJET Genomic DNA Purification Kit


Silica membrane-based genomic DNA purification from mammalian cell culture and tissue samples, whole blood, bacteria and yeast. Provided in a convenient spin-column format.
  
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The Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.

The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.

Highlights

  • Universal – can be used for both cell and tissue samples from a wide range of sources
  • Efficient – high yields of high molecular weight genomic DNA
  • Fast – 20 minute procedure following cell lysis
  • Convenient – spin columns are capped and assembled with collection tubes

Applications

  • Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
    • PCR and qPCR
    • FastDigest and conventional restriction digestion
    • Southern blotting

Includes

  • Proteinase K Solution
  • RNase A Solution
  • Digestion Solution
  • Lysis Solution
  • Wash Buffer I (concentrated)
  • Wash Buffer II (concentrated)
  • Elution Buffer
  • GeneJET Purification Columns (pre-assembled with collection tubes)
  • Collection tubes
  • Detailed Protocol
Table 1. | Typical genomic DNA yields from various sources.
Source Quantity Yield, µg
Mammalian blood 200 µL 4 to 6
Mouse heart 10 mg 10 to 15
Mouse tail 0.5 cm 8 to 10
Rat liver 10 mg 10 to 20
Rat spleen 5 mg 20 to 30
Rat kidney 10 mg 25 to 30
Rabbit ear 20 mg 5 to 10
Bacillus pumilis cells 2x109 cells 10 to 15
Escherichia coli cells 2x109 cells 10 to 15
HeLa cells 2x106 cells 15 to 20
Jurkat cells 5x106 cells 25 to 30
Saccharomyces cerevisiae cells 1x108 cells 3 to 5
  
Storage ConditionAmbient
HazardousNo
Quality Control
  • The GeneJET Genomic DNA Purification Kit is qualified by isolating genomic DNA from 200 µL of blood and 5 mg of mammalian tissue according to the recommended protocols.
  • The purified genomic DNA has an A260/280 ratio of ≥1.7.
  • A single band of more than 30 kb is seen after agarose gel electrophoresis and ethidium bromide staining.
  • Purified genomic DNA is evaluated by PCR amplification of a single-copy gene and digestion with FastDigest restriction enzymes.
Genomic DNA from various sources purified using GeneJET Genomic DNA Purification Kit

Genomic DNA from various sources purified using GeneJET Genomic DNA Purification Kit

Genomic DNA from various sources purified using GeneJET Genomic DNA Purification Kit

Figure 1. | Genomic DNA purified from various sources using the GeneJET Genomic DNA Purification Kit.
150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human bone marrow
2 – human blood
3 – rat spleen
4 – mouse heart
5 – mouse liver
6 – HeLa cells
7 – Jurkat cells
8 – E.coli DH5α cells


Yields of genomic DNA purified using GeneJET Genomic DNA Purification Kit and kits of competitors

Yields of genomic DNA purified using GeneJET Genomic DNA Purification Kit and kits of competitors

Yields of genomic DNA purified using GeneJET Genomic DNA Purification Kit and kits of competitors

Figure 2 | Yields of genomic DNA purified using the GeneJET Genomic DNA Purification Kit and kits from other vendors.


Overview of purification procedure

Overview of purification procedure

Overview of purification procedure

Overview of purification procedure.