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Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 µg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Figure 1. Superior purification of large and small DNA fragments from PCR reaction mixture.
Figure 2. Effective removal of unincorporated label.
Figure 3. Purified DNA can be used directly in sequencing reactions.A PCR fragment purified using the GeneJET PCR Purification Kit was sequenced using ABI PRISM® 377 DNA Analyzer. More than 650 nucleotides can be read.