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GeneJET Plant RNA Purification Mini Kit


Silica membrane-based total RNA purification from leaves, roots, sprouts and other parts of various plants. Provided in a convenient spin-column format.
  
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Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

Highlights

  • Fast – procedure completed in 20 min following cell lysis
  • Convinient – protocol does not include clogging-prone lysate filtration step
  • High yield – up to 65 µg of high purity and high integrity RNA from 50 mg plant sample
  • Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)

Applications

  • Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
    • RT-PCR
    • RT-qPCR
    • Northern blotting
    • Nuclease protection assay

Includes

  • Plant RNA Lysis Solution
  • Wash Buffer WB 1 (concentrated)
  • Wash Buffer 2 (concentrated)
  • Water, nuclease-free
  • GeneJET RNA Purification Columns (pre-assembled with collection tubes)
  • Collection tubes, 2 ml
  • Collection tubes, 1.5 ml
  • Detailed Protocol

Table 1. Typical RNA yelds from various sources.

Plant Tissue (50 mg) Yelds, µg
Arabidopsis thaliana leaf 17 to 20
Nicotiana tabacum leaf 17 to 21
Tomato leaf 50 to 53
Dandelion leaf 30 to 33
Spinach leaf 25 to 30
Corn leaf 25 to 30
seeds 6 to 7
Sugar-cane leaf 4 to 5
Rape leaf 25 to 30
roots 20 to 22
seeds 40 to 42
Onion leaf 10 to 11
Lettuce leaf 10 to 11
Lucerne leaf 35 to 40
Cucumber fruit 40 to 45
Lemon leaf 5 to 7
Rice leaf 20 to 25
Wheat leaf 60 to 65
Sunflower stalk 10 to 11
seeds 10 to 12
Sugar-beet roots 6 to 7
Soy seeds 5 to 7
  
Storage ConditionAmbient
HazardousNo
Quality Control
  • The GeneJET Plant RNA Purification Mini Kit is qualified by isolating total RNA from 50 mg of plant tissue following the protocol outlined in the manual.
  • The quality of isolated RNA is evaluated spectrophotometrically and by agarose gel electrophoresis.
  • The purified RNA has an A260/280 ratio between 1.9 and 2.1 and the RNA integrity number (RIN) of ≥7.
Overview of purification procedure

Overview of purification procedure

Overview of purification procedure

Figure 1. | Overview of purification procedure


High integrity RNA isolated from difficult to process samples using GeneJET Plant RNA Purification Mini Kit.

High integrity RNA isolated from difficult to process samples using GeneJET Plant RNA Purification Mini Kit.

High integrity RNA isolated from difficult to process samples using GeneJET Plant RNA Purification Mini Kit.

Figure 2. | High integrity RNA isolated from difficult to process samples using GeneJETPlant RNA Purification Mini Kit. 1 µL of total RNA isolated from 50 mg of various plants and plant parts analyzed using Agilent 2100 Bioanalyzer™.

1 – Corn roots

2 – Rape roots

3 – Sunflower stalk

4 – Rape stalk

5 – Cucumber fruit

6 – Tomato fruit

7 – Sunflower seeds

8 – Rape seeds

9 – Corn seeds


RNA purified from tomato leaves using different kits

RNA purified from tomato leaves using different kits

RNA purified from tomato leaves using different kits

Figure 3. | RNA purified from tomato leaves using different kits. 1 µL (2% of eluate) of RNA isolated using the GeneJET Plant RNA Purification Mini Kit and the kits from other vendors were fractionated on 1% agarose gel.

M – RiboRuler High Range RNA Ladder (#SM1821)

1 – Thermo Scientific

2-5 – various vendors