The Thermo Scientific GeneJET Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Yields of high-copy plasmid DNA obtained using GeneJET Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial culture volumes.
Concentrations of eluted DNA obtained after the purification of high-copy plasmid DNA using different kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.