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The Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Figure 1 | Overview of purification procedure.
Figure 2 | Total RNA from various sources purified using the GeneJET RNA Purification Kit. 1 µg of isolated total RNA was analyzed by agarose gel (1%) electrophoresis. M – RiboRuler High Range RNA Ladder (#SM1821) 1 – mouse liver 2 – mouse spleen 3 – HeLa cells 4 – Cos-7 cells 5 – Saccharomyces cerevisiae 6 – Escherichia coli DH5α
Figure 3 | Yields of total RNA purified using the GeneJET RNA Purification Kit and kits of competitors.
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R. Boom, C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. W. Dillen, J. van der Noordaa, Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28(3), 495–503 (March 1990).