Previously added items:
The Thermo Scientific GeneJET Viral DNA and RNA Purification Kit is designed for rapid and efficient purification of high quality viral nucleic acids from various human and mammalian liquid samples, and is compatible with common sample preservatives like EDTA, citrate, and heparin.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column. Viral nucleic acids from lysed samples bind to the column membrane while impurities are effectively removed during subsequent washing and centrifugation steps. Ready-to-use nucleic acids are eluted from the column.
The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA and RNA can be directly used in PCR, qPCR, or other nucleic acid based assays.
Figure 1. |
Figure 2. | Real-time PCR detection of Viral RNA (A) and DNA (B) purified with the GeneJET Viral DNA and RNA Purification Kit. Samples of various HBV and HCV titers were produced by diluting infected plasma of known viral titer with non-infected plasma. A: Detection of RNA from various HCV sample titers. B: Detection of DNA from various HBV sample titers.
Figure 3. | Different amounts of HBV, HCV, CMV, and EBV infected human plasma or blood samples were purified using GeneJET Viral DNA and RNA Purification kit, and using kits from other suppliers. Isolated viral nucleic acids were quantified using a real-time PCR detection assay.
Figure 4. | Isolation of viral nucleic acids from multiple replicates of two plasma samples infected with high and low HCV (A) or HBV (B) titers using the GeneJET Viral DNA and RNA Purification Kit. Isolated HCV and HBV nucleic acids were quantified using a real-time PCR detection assay. A: Detection of RNA from multiple replicates of HCV plasma samples with high and low viral titers. B: Detection of DNA from multiple replicates of HBV plasma samples with high and low viral titers.