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GeneJET Whole Blood Genomic DNA Purification Mini Kit


Silica membrane-based genomic DNA purification from fresh, frozen anticoagulated mammalian blood, aged blood, buffy coat, bone marrow, body fluids. Provided in a convenient spin-column format.
  
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The Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Highlights

  • Efficient – high yields of high molecular weight genomic DNA
  • Fast – 20 minute procedure following cell lysis
  • Convenient – spin columns are capped and assembled with collection tubes
  • Pure – DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
  • Compatible – suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids

Applications

  • Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
    • PCR and qPCR
    • FastDigest and conventional restriction digestion
    • Southern blotting

Includes

  • Proteinase K Solution
  • Lysis Solution
  • Wash Buffer WB I (concentrated)
  • Wash Buffer II (concentrated)
  • Elution Buffer
  • GeneJET Purification Columns (pre-assembled with collection tubes)
  • Collection tubes
  • Detailed Protocol
  
Storage ConditionAmbient
HazardousNo
Quality Control
  • The GeneJET Whole Blood Genomic DNA Purification Mini Kit is qualified by isolating genomic DNA from 200 µL of blood following the protocols outlined in the manual.
  • The purified genomic DNA has an A260/280 ratio between 1.7 and 1.9.
  • A single band of more than 30 kb is observed after agarose gel electrophoresis and ethidium bromide staining.
  • The functional quality of purified genomic DNA is evaluated by PCR amplification of a single-copy gene and by digestion with restriction enzymes.
Overview of Purification Process

Overview of Purification Process

Overview of Purification Process


Genomic DNA purified from various sources using the GeneJET Whole Blood Genomic DNA Purification Mini Kit

Genomic DNA purified from various sources using the GeneJET Whole Blood Genomic DNA Purification Mini Kit

Genomic DNA purified from various sources using the GeneJET Whole Blood Genomic DNA Purification Mini Kit

200 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human whole blood
2 – human buffy coat
3 – human bone marrow
4 – avian whole blood. 200 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis
M – Lambda DNA/HindIII Marker (#SM0101)
1 – human whole blood
2 – human buffy coat
3 – human bone marrow
4 – avian whole blood


Accurate evaluation of genomic DNA yields

Accurate evaluation of genomic DNA yields

Accurate evaluation of genomic DNA yields

Genomic DNA was purified from human blood using the GeneJET Whole Blood Genomic DNA Purification Mini Kit and kits from other vendors. DNA concentration was estimated using Nanodrop spectrophotometer (Thermo Scientific) and 200 pg used as template in qPCR with primers against single copy gene. Matching yields obtained by qPCR based absolute quantification and spectrophotometrical evaluation indicate absence of reaction inhibitors and contaminating RNA.