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The Thermo Scientific Viral DNA/RNA Purification Kit is a simple and easy-to-use system for efficient purification of viral nucleic acids from human plasma or serum samples for in vitro diagnostic purposes.
The purification system utilizes silica-based membrane technology in the convenient form of a spin column. The entire purification procedure takes approximately 30 minutes to process 1 to 4 samples. Purified viral DNA and RNA are compatible with downstream qualitative and quantitative in vitro diagnostic workflows, such as PCR, qPCR, RT-PCR, and RT-qPCR. The kit is compatible with citrate- or EDTA-treated human plasma and serum samples. To avoid sample contamination and to ensure safe waste handling, fresh wash tubes are supplied for each wash step.
During the purification procedure, viral nucleic acids from lysed plasma or serum samples bind to the column membrane while impurities are effectively removed during subsequent wash steps. Ready-to-use nucleic acids are then eluted from the column. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications.
Figure 2.Real-time PCR detection of Viral RNA (A) and DNA (B) purified with the Viral DNA/RNA Purification Kit. Samples of various HBV and HCV titers were produced by diluting infected plasma of known viral titer with non-infected plasma.
Figure 3.Isolation of viral nucleic acids from multiple replicates of two plasma samples infected with high and low HCV (A) or HBV (B) titers using the Viral DNA/RNA Purification Kit. Isolated HCV and HBV nucleic acids were quantified using a real-time PCR detection assay.