DreamTaq Green DNA Polymerase

Available On-Site


Enhanced Taq DNA polymerase supplied with colored buffer enabling direct gel loading and significant time savings. Optimized for all standard PCR applications.
  
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Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.

DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Highlights

  • Direct loading of PCR product on gel
  • High yields and high sensitivity of PCR
  • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
  • Robust amplification with minimal optimization
  • Incorporates modified nucleotides, but does not incorporate dUTP

Applications

  • Routine PCR amplification of DNA fragments up to 6 kb
  • Genotyping
  • High throughput PCR

Includes

DreamTaq Green DNA Polymerase:

  • DreamTaq Green DNA Polymerase
  • 10X DreamTaq Green Buffer (includes 20 mM MgCl2)

DreamTaq Green PCR Master Mix (2X):

  • DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
  • Nuclease-free water
  
10X DreamTaq Green BufferA proprietary formulation which, in addition to the PCR buffer components, includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress. The dyes have excitation peaks at 424 nm and 615 nm.

The 10X DreamTaq Green Buffer contains KCl and (NH4)2SO4 at a ratio optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.
Definition of Activity Unit
  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
  • Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
HazardousNo
InactivationInactivated by phenol/chloroform extraction.
InhibitionInhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1).
Quality ControlThe absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in PCR.
Storage BufferThe enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, stabilizing agent and 50% (v/v) glycerol.
Storage Condition-20 C
Longer PCR fragments and higher yields with DreamTaq Green DNA Polymerase

Longer PCR fragments, higher yields with DreamTaq Green DNA Polymerase

Longer PCR fragments and higher yields with DreamTaq Green DNA Polymerase

Amplification of PCR fragments from complex mouse and human genomic DNA using DreamTaq Green DNA Polymerase and other commercial enzymes that allow direct gel loading of PCR mixtures. PCR was performed according to supplier recommendations in a 50 µL reaction volume and 10 µL of each PCR mixture was loaded on the gel.

M – GeneRuler Express DNA Ladder
1 – 424 bp lcs gene fragment from mouse genomic DNA
2 – 956 bp 7 chromosome STS (G31656) from human genomic DNA
3 – 2537 bp tPA gene fragment from human genomic DNA
4 – 5003 bp beta-globine gene fragment from human genomic DNA


References

  1. R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9(3), 308-309 (September 1990).

Citations