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Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.
DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
Amplification of PCR fragments from complex mouse and human genomic DNA using DreamTaq Green DNA Polymerase and other commercial enzymes that allow direct gel loading of PCR mixtures. PCR was performed according to supplier recommendations in a 50 µL reaction volume and 10 µL of each PCR mixture was loaded on the gel.M – GeneRuler Express DNA Ladder1 – 424 bp lcs gene fragment from mouse genomic DNA2 – 956 bp 7 chromosome STS (G31656) from human genomic DNA3 – 2537 bp tPA gene fragment from human genomic DNA 4 – 5003 bp beta-globine gene fragment from human genomic DNA
R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9(3), 308-309 (September 1990).