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DyNAzyme I & II DNA Polymerases


DyNAzyme DNA Polymerases are Taq-like enzymes with improved thermal stability at high temperatures.
  
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Thermo Scientific DyNAzyme DNA Polymerases are Taq-like enzymes with improved thermal stability at high temperatures. They are ideal for all routine PCR applications, and have improved tolerance against DMSO. DyNAzyme I DNA Polymerase is a native thermostable DNA polymerase from Thermus brockianus. DyNAzyme II DNA Polymerase is an easy-to-use, recombinant thermostable DNA polymerase for standard PCR.

Highlights

  • Improved thermal stability
  • Improved tolerance against DMSO
  • Supplied with optimized buffers or with Mg2+-free buffers and MgCl2 solution
  • Detergent-free buffer also available

Applications

  • Routine PCR
  • Microarray and DHPLC

Includes

DyNAzyme I DNA Polymerase Kit (F-550):

  • DyNAzyme I DNA Polymerase 2 U/µL
  • Optimized DyNAzyme buffer
  • Mg2+-free DyNAzyme buffer
  • dNTP Mix 10 mM each
  • MgCl2 50 mM
  • Control lambda template 0.5 ng/µL
  • 500 bp control primers 25 µM each
  • DNA size standard

DyNAzyme II DNA Polymerase (F-501):

  • DyNAzyme II DNA Polymerase 2 U/µL
  • 10X Optimized DyNAzyme Buffer (1X buffer contains: 10 mM Tris-HCl (pH 8.8 at 25°C), 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton

DyNAzyme II DNA Polymerase with Mg2+-free DyNAzyme Buffer and MgCl2 Solution (F-503):

  • DyNAzyme II DNA Polymerase 2 U/µL
  • 10X Mg2+-free DyNAzyme Buffer
  • 50 mM MgCl2 solution
  
Storage Condition-20 C
HazardousNo

Citations

Polymerase structure, Basic articles

  1. T. Kasuga, J. Cheng, K. Mitchelson, Metastable Single-strand DNA Conformational Polymorphism Analysis Results in Enhanced Polymorphism Detection. PCR Methods and Applications. 4, 227-233 (1995).

  2. E. Rytkönen et al., The human gene for xanthine dehydrogenase (XDH) is localized on chromosome band 2p22. Cytogenet Cell Genet. 68, 61-63 (1995).

  3. K. Huoponen et al., Time-Resolved Fluorometry in the Diagnosis of Leber Hereditary Optic Neuroretinopathy. Human Mutation. 3, 29-36 (1994).

  4. Q. He et al., Primers and Decisive for Sensitivity of PCR. BioTechniques.  17(1), 82-84 (1994).

  5. K. Huoponen et al., The spectrum of mitochondrial DNA mutations in families with Leber hereditary optic neuroretinopathy. Human Genet. 92, 379-384 (1993)

  6. M. J. Longley, D. W. Mosbaugh, Characterization of DNA metabolizing enzymes in situ  following polyacrylamide gel electrophoresis. Biochemistry. 30, 2655-2664 (1991).