DreamTaq DNA Polymerase


DreamTaq DNA Polymerase
Enhanced Taq DNA polymerase optimized for all standard PCR applications ensuring higher sensitivity, longer PCR products and higher yields.
  
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Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl2.

DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Highlights

  • Robust amplification with minimal optimization
  • High yields of PCR products
  • Higher sensitivity compared to conventional Taq DNA polymerase
  • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
  • Incorporates modified nucleotides, but does not incorporate dUTP

Applications

  • Routine PCR amplification of DNA fragments up to 6 kb
  • High throughput PCR
  • Genotyping

Includes

DreamTaq DNA Polymerase:

  • DreamTaq DNA Polymerase 5 U/µL
  • 10X DreamTaq Buffer (includes 20 mM MgCl2)

DreamTaq PCR Master Mix (2X):

  • DreamTaq DNA Polymerase, 2X DreamTaq buffer, dNTPs, and 4 mM MgCl2
  • Nuclease-free water
  
Definition of Activity Unit
  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
  • Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
HazardousNo
InactivationInactivated by phenol/chloroform extraction
InhibitionInhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1)
Quality Control
  • The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested in PCR.
Storage BufferThe enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, stabilizing agent, and 50% (v/v) glycerol.
Storage Condition-20 C
Comparison of PCR sensitivity and yield

Comparison of PCR sensitivity and yield with DreamTaq DNA Polymerase and other commercial Taq DNA Polymerases

Comparison of PCR sensitivity and yield

A 545 bp fragment from the human α-L-Fucosidase gene was amplified according to manufacturers’ recommendations using decreasing amounts of human genomic DNA.

MFastRuler Low Range DNA Ladder, ready-to-use
DDreamTaq DNA Polymerase
TTaq DNA Polymerase
1 through 3Taq DNA polymerases from different vendors


References

  1. R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9(3), 308-309 (September 1990).

Citations