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Long PCR Enzyme Mix

Not available in the USA

Store at -20°C

A unique enzyme blend for long and tricky PCR applications.
  
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Thermo Scientific Long PCR Enzyme Mix is a unique blend of Taq DNA Polymerase and a thermostable high fidelity DNA polymerase with proofreading activity. The two enzymes synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone.

The fidelity of PCR with this enzyme mix is three times higher than with Taq DNA Polymerase. The ratio of enzymes in the Long PCR Enzyme Mix is optimized for generation of very long amplicons: up to 47 kb with viral DNA and up to 21 kb with genomic DNA templates. The specially formulated Long PCR Buffer protects DNA from depurination and nicking during long thermal cycling. The products generated with the Long PCR Enzyme Mix are mostly 3'-dA tailed. Long PCR Enzyme Mix is also used for efficient amplification of GC-rich DNA regions.

Highlights

  • Unique blend of two thermostable DNA polymerases
  • Long PCR products:
    • up to 47 kb with viral DNA as template
    • up to 21 kb with genomic DNA as template
  • Ideal for GC-rich templates up to 85%
  • Three times higher fidelity compared to Taq DNA polymerase
  • High yield

Applications

  • Long range PCR
  • PCR from GC-rich or difficult templates

Includes

  • Long PCR Enzyme Mix (5 U/µL)
  • 10X Long PCR Buffer with 15 mM MgCl2
  • 10X Long PCR Buffer
  • 25 mM MgCl2
  • DMSO
  • Nuclease-free water
  
Storage Condition-20 C
HazardousNo
Quality ControlFunctionally tested in generation of 47.4 kb amplicon with lambda DNA as template.
Amplification of long DNA fragments

Amplification of long DNA fragments

Amplification of long DNA fragments

DNA fragments were amplified from 1.25 ng of lambda DNA in 50 µL of reaction mixture containing 2.5 U of Long PCR Enzyme Mix.

M – Lambda Mix Marker
1 to 5 – PCR products


Successful amplification of GC-rich template with the Long PCR Enzyme Mix

Successful amplification of GC-rich template with the Long PCR Enzyme Mix

Successful amplification of GC-rich template with the Long PCR Enzyme Mix

Human IGFR II gene (85% GC content) was amplified using the Long PCR Enzyme Mix and two other commercial PCR systems for GC-rich templates. PCR mixtures (50 µL) contained 100 ng of human genomic DNA, 200 µM each dNTP and 0.5 µM of each primer. Cycling conditions: 94°C, 2 min; 96°C, 10 s; 68°C, 1 min 30 s; 35 cycles; 72°C, 7 min.

M – GeneRuler 100 bp Plus DNA Ladder
1 – Long PCR Enzyme Mix supplemented with 12% DMSO
2 to 3 – PCR systems for GC-rich templates from other vendors


Product Guides and Selectors

pdf   PCR Reagents Selection Guide