Maxima Hot Start Green PCR Master Mix

A - Unseparated in well

B - Blue and yellow dyes after gel electrophoresis

Thermo Scientific Maxima Hot Start Green PCR Master Mix (2X) is a ready-to-use solution containing Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg²⁺, and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel.

The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of Maxima Hot Start Taq DNA Polymerase. It is capable of amplification of up to 3 kb from genomic DNA.

Highlights

• Convenient – Maxima Hot Start Taq DNA Polymerase in a ready-to-use mix
• Hot start PCR – reaction set-up at room temperature
• Four minute activation time
• Direct loading of PCR product on gels
• High specificity and sensitivity of PCR

Applications

• Hot start PCR
• Routine PCR
• High throughput PCR
• Multiplex PCR
• Genotyping

Includes

• Maxima Hot Start Taq DNA polymerase, 2X hot start PCR buffer, 0.4 mM of each dNTP, 4 mM Mg²⁺, density reagent, and two dyes for direct loading on agarose gels.
• Nuclease-free water