Previously added items:
A - Unseparated in well
B - Blue and yellow dyes after gel electrophoresis
Thermo Scientific Maxima Hot Start Green PCR Master Mix (2X) is a ready-to-use solution containing Maxima Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg2+, and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel.
The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of Maxima Hot Start Taq DNA Polymerase. It is capable of amplification of up to 3 kb from genomic DNA.
Amplification of 956 bp single copy gene from 0.1 µg human genomic DNA using PCR Master Mix (2X) in 50 µ: reaction mixture. M – GeneRuler Express DNA Ladder (#SM1551) 1, 2 – PCR product generated with 2 U of Maxima Hot Start Taq DNA Polymerase3, 4 – PCR product generated with Maxima Hot Start Green PCR Master Mix (2X) stored at -20°C5, 6 – PCR product generated with Maxima Hot Start Green PCR Master Mix (2X) after 25 freeze-thaw cycles