Maxima Hot Start Taq DNA Polymerase

Available On-Site

Store at -20°C Not inactivated at 80°C in 20 min

Hot start DNA polymerase designed to enhance the specificity, sensitivity, and yield of DNA amplification.
  
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Thermo Scientific Maxima Hot Start Taq DNA Polymerase is a chemically modified recombinant Taq DNA polymerase. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. Maxima Hot Start Taq DNA Polymerase is also available in a master mix format.

Highlights

  • Four minute activation time
  • High PCR specificity and sensitivity
  • Room temperature PCR set up

Applications

  • Hot Start PCR
  • Routine PCR
  • High throughput PCR
  • Multiplex PCR
  • Genotyping

Includes

Maxima Hot Start Taq DNA Polymerase:

  • Maxima Hot Start Taq DNA Polymerase (5 U/µL)
  • 10X Hot Start PCR Buffer
  • 25 mM MgCl2

Maxima Hot Start PCR Master Mix (2X):

  • Maxima Hot Start Taq DNA polymerase, 2X Hot Start PCR buffer, 0.4 mM of each dNTP and 4 mM Mg2+.
  • Nuclease-free water
  
10X Hot Start PCR Buffer200 mM Tris-HCl (pH 8.3 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4.
Definition of Activity Unit
  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 74°C.
  • Enzyme activity is assayed in the following mixture: 25 mM TAPS (pH 9.3 at 25°C), 50 mM KCl, 2 mM MgCl2, 0.2 mM of each dATP, dGTP, dTTP, 0.1 mM dCTP, 0.75 mM activated salmon milt DNA, and 0.4 MBq/mL [3H]-dCTP.
HazardousNo
InactivationInactivated by phenol/chloroform extraction.
InhibitionInhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1).
Molecular Weight94 kDa monomer
Quality Control
  • The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested in hot-start PCR.
Storage BufferThe enzyme is supplied in 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween 20, 0.5% (v/v) Nonidet P40, and 50% (v/v) glycerol.
Storage Condition-20 C
Thermo Scientific Maxima Hot Start Taq yields more product or greater specificity than two competitors, as shown via agarose gel electrophoresis.

Specific amplification with Maxima Hot Start Taq DNA Polymerase

Thermo Scientific Maxima Hot Start Taq yields more product or greater specificity than two competitors, as shown via agarose gel electrophoresis.

50 ng of human genomic DNA was used as a template for amplification of a 2 kb fragment of the beta-globine gene using hot start Taq DNA polymerases from different vendors in 35 cycles on the GeneAmp 9700 PCR System.

M – GeneRuler 100 bp Plus DNA Ladder
1 – Maxima Hot Start Taq DNA Polymerase
2 – Hot start Taq DNA Polymerase (chemical modification), Vendor A
3 – Hot start Taq DNA Polymerase (temperature-dependent inhibitor), Vendor B


References

  1. R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9(3), 308-309 (September 1990).

Citations