Thermo Scientific Maxima Hot Start Taq DNA Polymerase is a chemically modified recombinant Taq DNA polymerase. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4 minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase. Maxima Hot Start Taq DNA Polymerase is also available in a master mix format.
Maxima Hot Start Taq DNA Polymerase:
Maxima Hot Start PCR Master Mix (2X):
50 ng of human genomic DNA was used as a template for amplification of a 2 kb fragment of the beta-globine gene using hot start Taq DNA polymerases from different vendors in 35 cycles on the GeneAmp 9700 PCR System.M – GeneRuler 100 bp Plus DNA Ladder1 – Maxima Hot Start Taq DNA Polymerase2 – Hot start Taq DNA Polymerase (chemical modification), Vendor A3 – Hot start Taq DNA Polymerase (temperature-dependent inhibitor), Vendor B
R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9(3), 308-309 (September 1990).