Home | Molecular Biology | PCR, qPCR & Reverse Transcription | PCR Enzymes, Master Mixes & Reagents | Standard PCR

Taq DNA Polymerase, Native

Low concentration available Store at -20°C Not inactivated at 80°C in 20 min

Highly thermostable, native DNA polymerase for routine PCR.
  
Loading...

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Taq DNA Polymerase (native, with BSA) is supplied with BSA as a stabilizing agent. This version of Taq DNA Polymerase is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Highlights

  • Thermostable – half life is more than 40 min at 95°C.
  • Generates PCR products with 3’-dA overhangs.
  • Supplied with two buffers – 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • DNA labeling (see References 1-3)
  • High throughput PCR

Includes

Taq DNA Polymerase (native, with BSA):

  • Taq DNA Polymerase 5 U/µL
  • 10X Taq Buffer with KCl
  • 10X Taq Buffer with (NH4)2SO4
  • 25 mM MgCl2
  • 20 mg/mL BSA

Taq DNA Polymerase (native, without BSA):

  • Taq DNA Polymerase 5 U/µL
  • 10X Taq Buffer with KCl
  • 10X Taq Buffer with (NH4)2SO4
  • 25 mM MgCl2

Taq DNA Polymerase (native, without BSA), LC:

  • Taq DNA Polymerase 1 U/µL
  • 10X Taq Buffer with KCl
  • 10X Taq Buffer with (NH4)2SO4
  • 25 mM MgCl2

Note

  • The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in (see Reference 4). Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
  • The 10X Taq Buffer without Detergent is recommended for microarray experiments.
  
Storage Condition-20 C
HazardousNo
10X Taq Buffer with (NH4)2SO4750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20.
10X Taq Buffer with KCl100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40.
Definition of Activity Unit
  • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
  • Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl(pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
InactivationInactivated by phenol/chloroform extraction.
InhibitionInhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02, and 0.01%, respectively (5).
Molecular Weight94 kDa monomer
Quality Control
  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested in PCR.
SourceThermus aquaticus YT1 cells
Storage BufferThe enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycerol.

References

  1. D. Celeda et al., PCR amplification and simultaneous digoxigenin incorporation of long DNA probes for fluorescence in situ hybridization. BioTechniques. 12, 98-102 (1992).

  2. U. Finckh et al., Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol. BioTechniques. 10, 35-39 (1991).

  3. H. Yu et al., Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Res. 22, 3226-3232 (1994).

  4. K. S. Lundberg et al., High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 108(1), 1-6 (1 December 1991).

  5. R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9, 308-309 (1990).

Product Guides and Selectors

pdf   PCR Reagents Selection Guide