Home | Molecular Biology | PCR, qPCR & Reverse Transcription | PCR Enzymes, Master Mixes & Reagents | Direct PCR

Phire Plant Direct PCR Kit


Efficient PCR directly from different plant materials across species.
  
Loading...

PlantThermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material.

Phire Plant Direct PCR Kit has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, sampling tools and detailed protocols making it an ideal choice for amplification of plant DNA.When combined with Piko Thermal Cycler and Piko UTW PCR Plates, PCR protocols can be completed in as little as 30 minutes.

Phire Plant Direct PCR Kit

Highlights

  • No need for time-consuming and expensive DNA purification steps
  • Very little sample material required
  • Two simple protocols for various applications
  • Convenient sampling tools included in the kit
  • Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/ kb).

Applications

  • Genotyping
  • Transgene detection

Includes

  • Phire Hot Start II DNA Polymerase
  • 2X Phire Plant PCR Buffer (includes dNTPs and MgCl2)
  • Control Primer Mix (25 µM each)
  • Dilution Buffer
  • Harris Uni-Core™ 0.50 mm
  • Harris Cutting Mat™

Two short Protocols for various needs

In the direct protocol, a small piece of plant material is placed directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons, and for assays where several DNA fragments are amplified from the same sample.

  
Storage Condition-20 C
HazardousNo
Phire Plant Direct PCR Kit allows efficient PCR from various plant leaves and seeds

Phire Plant Direct PCR Kit allows efficient PCR from various plant leaves and seeds

Phire Plant Direct PCR Kit allows efficient PCR from various plant leaves and seeds

Various amplicons from mitochondrial or genomic DNA were succesfully amplified with Phire Plant Direct PCR Kit. 0.50 mm punches from different leaves were placed directly into 50 µL PCR reactions. Various amplicons from mitochondrial or genomic DNA were successfully amplified with the Phire Plant Direct PCR Kit. + and - denote control reactions with or without purified DNA.

1 – Arabidopsis, mtDNA (1 kb)
2 – Arabidopsis, gDNA (3.5 kb)
3 – Capsicum, mtDNA (1.4 kb)
4 – Maize, mtDNA (1.4 kb)


Detection of RNAi vector in gerbera plants using Phire Plant Direct PCR Kit

Detection of RNAi vector in gerbera plants using Phire Plant Direct PCR Kit

Detection of RNAi vector in gerbera plants using Phire Plant Direct PCR Kit

Punches (0.50 mm) from plant leaves or petal tissues were placed directly in 20 µL PCR reactions. 210 bp products were amplified using transgene specific primers. The results indicate that plant individuals corresponding to samples 2, 4, 7, 9, and 11 contain the RNAi transgene. The obtained results were confirmed by conventional analysis of CTAB DNA extraction followed by PCR. 1 through 9 – Leaves. 10 through 12 – Petals.


Citations

  1. L. W. Zhou, Y. L. Wei, Changbai wood-rotting fungi 16. A new species of Fomitopsis (Fomitopsidaceae). Mycol Progress. 10:1007/s11557-011-0758-x (2011).
  2. N. Bergmann et al., Population-specificity of heat stress gene induction in northern and southern eelgrass Zostera marina populations under simulated global warming. Mol. Ecol. 19, 2870-2883 (2010).
  3. G. S. Khew, T. F. Chia, Parentage determination of Vanda Miss Joaquim (Orchidaceae) through two chloroplast genes rbcL and matK. AoB PLANTS. 2011, plr018 doi:10.1093/aobpla/plr018 (16 August 2011).