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Thermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood.
This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Kit an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Kit also allows analysis of blood samples with different anticoagulants, storage conditions and from various species.
Amplification of a 237 bp DNA fragment directly from whole blood of seven different vertebrates (5% blood in the reaction). + and - denote control reactions
Amplification of three DNA fragments from human blood collected on various filter papers. Piko Thermal Cycler and UTW reaction vessels were used in PCR. + and - denote control reactions.1 – 903 Card2 – FTA Gene Card3 – FTA Elute Card
The Phusion Blood Direct PCR Kit was compared to other kits designed for PCR directly from blood. A 588 bp genomic DNA fragment was amplified in the presence of increasing blood concentration in the reaction mixture. PCR was performed according to suppliers instructions using Piko Thermal Cycler and ultra-thin walled UTW reaction vessels. Total protocol times indicated at bottom. + and - denote control reactions.
H-P. Fuehrer et al., Novel Nested Direct PCR Technique for Malaria Diagnosis Using Filter Paper Samples. J. Clin. Microbiol. 49, 1628-1630 (2011).
N. Awad, A. E. El-Tarras, Genetic Analysis of Protein Phophatase 1 Regulatory Subunit 1 B (PPP1R1B) Gene among Autistic Patients. Aust. J. Basic & Appl. Sci. 5, 1-5 (2011).