Previously added items:
A 950 bp single copy gene from human genomic DNA was amplified at a wide range of Mg2+ concentrations.M – GeneRuler 100 bp Plus DNA Ladder
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable 3’→5’ exonuclease (proofreading) activity and possesses low 5’→3’ exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Taq DNA Polymerase (recombinant):
Taq DNA Polymerase (recombinant), LC:
PCR Master Mix (2X):
D. Celeda et al., PCR amplification and simultaneous digoxigenin incorporation of long DNA probes for fluorescence in situ hybridization. BioTechniques. 12, 98-102 (1992).
U. Finckh et al., Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol. BioTechniques. 10, 35-39 (1991).
H. Yu et al., Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Res. 22, 3226-3232 (1994).
K. S. Lundberg et al., High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 108, 1-6 (1991).
R. S. Weyant et al., Effect of ionic and nonionic detergents on the Taq polymerase. BioTechniques. 9, 308-309 (1990).