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Thermo Scientific TrueStart Hot Start Taq DNA Polymerase is designed for hot start PCR, a technique shown to improve specificity, sensitivity and yield of DNA amplification during PCR (see References 1,2,3,4,5). TrueStart Hot Start Taq DNA Polymerase is chemically modified by adding proprietary heat-labile blocking groups to amino acid residues. TrueStart Hot Start Taq DNA Polymerase is inactive at room temperature because of this modification, avoiding extension of non-specifically annealed primers or primer dimers.
TrueStart Hot Start Taq DNA Polymerase activates rapidly during the initial denaturation step of PCR. The lack of additional activation step differentiates it from other hot start DNA polymerases, making this enzyme an ideal tool for both automatic hot start PCR and fast PCR. Activated TrueStart Hot Start Taq DNA Polymerase catalyzes 5'→3' synthesis of DNA, lacks detectable 3'→5' exonuclease (proofreading) activity, but possesses low 5'→3' exonuclease activity. These two activities are not detectable before activation.
A 2 kb DNA fragment of the human beta-globine gene was amplified according to the manufacturer’s recommendations.M – GeneRuler100 bp Plus DNA Ladder1 to 2 – TrueStart Hot Start Taq DNA Polymerase3 to 4 – Taq DNA Polymerase (chemical modification), Vendor A5 to 6 – Taq DNA Polymerase (temperature-dependent inhibitor), Vendor B7 to 8 – Taq DNA Polymerase (antibody-based), Vendor C9 to 10 – Taq DNA Polymerase (not hot start), Thermo Scientific
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