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Direct PCR

Direct PCR  

Direct PCR Kits

 

The Thermo Scientific Direct PCR approach delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified samples. Significant savings in time and cost can be achieved in applications such as plant and animal genotyping. Learn more about the Direct PCR method.

 
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Phusion
Human Specimen
Direct PCR Kit  

Phusion Human Specimen Direct PCR Kit contains a complete set of optimized reagents for various sample types, such as buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth and skin biopsies.

 
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Phire Animal Tissue Direct PCR Kit  

Phire Animal Tissue Direct PCR Kit is a complete kit for for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

 
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Phire Plant
Direct PCR Kit  

Phire Plant Direct PCR Kit includes a complete set of optimized reagents, sampling tools and control primers for convenient amplification of plant DNA.

 
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Phusion Blood
Direct PCR Kit  

Phusion Blood Direct PCR Kit is designed for amplification of DNA directly from whole blood.

 
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Direct PCR accessories:
Harris Uni-Core
and Cutting Mat  

The Harris Uni-Core is a convenient tool for cutting very small punch discs from materials such as animal tissue, plant leaves or Whatman FTA or Whatman 903 cards for Direct PCR. A compatible Harris Cutting Mat is used to provide an even cutting surface for the Harris Uni-Core.

 

Direct PCR is based on Thermo Scientific Phusion High-Fidelity DNA Polymerases and Thermo Scientific Phire Hot Start II DNA Polymerase.They are exceptionally tolerant of many PCR inhibitors present in various sample material. This allows robust amplification in conditions where conventional DNA polymerases are completely inhibited. Direct PCR provides two alternative protocols for various needs.

DirectPCR Workflow