Spectra Multicolor Broad Range Protein Ladder

Available On-Site


A prestained, 4-color, SDS-PAGE MW marker especially for proteins between 10 and 260kDa.
  
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The following bands are visible: ~260 kDa (orange), ~140 kDa (pink), ~100 kDa (blue), ~70 kDa (orange), ~50 kDa (green), ~40 kDa (orange), ~35 kDa (blue), ~25 kDa (blue), ~15 kDa (blue), and ~10 kDa (green).

Images are from a 4-12% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.

The Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained recombinant prokaryotic proteins (10 to 260 kDa) for use as size standards in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins (1-3) that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.

Highlights

  • Size range – 10 proteins spanning 10 to 260 kDa
  • Multicolor – four different colors for unambiguous band-size assignment
  • Ready-to-use – supplied in a loading buffer for direct loading on gels, no need to boil
  • Sharp bands – color-coded bands of similar intensity for easy visualization
  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting
  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Applications

  • Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
  • Monitoring protein transfer onto membranes after Western blotting
  • Sizing of proteins on SDS-polyacrylamide gels and Western blots

Note

For migration patterns under different gel conditions, see Specifications.

  
HazardousNo
Quality ControlTested in SDS-polyacrylamide gel electrophoresis and Western Blotting.
StorageStable at 4°C for up to 3 months. For long term storage, store at -20°C.
Storage Buffer62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, and 33% glycerol.

Migration patterns of Spectra Multicolor Broad Range Protein Ladder in different electrophoretic conditions

Migration pattern of Spectra Multicolor Low Range Protein Ladder in Tris-Tricine, Tris-Acetate, and Bis-Tris gels

The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.