PikoReal Real-Time PCR System

Real time, any time - Now within your reach

PikoReal Real-Time PCR System
The PikoReal Real-Time PCR System is designed to fit into your lab, even when bench-top space is limited. It is ideal for personal use and light enough for field applications.

The Thermo Scientific PikoReal Real-Time PCR System is a highly flexible gene quantification and genotyping platform in 24- and 96-well block formats, and is designed with a minimal footprint, making it ideal for personal benchtop use and field applications.

The PikoReal Real-Time PCR System incorporates innovative, energy-saving technologies to achieve fast performance while meeting the highest thermal requirements – all with reduced energy, plastics, and reagent consumption for real cost savings. It also uses Ultra Thin Wall (UTW) Piko PCR plates that are 25% the size of conventional plates, yet compatible with multi-channel pipettes, reagent dispensers, and automated liquid handling systems.


  • Minimal footprint for personal benchtop use
  • Five detection channels enable up to four target multiplexing
  • High temperature uniformity across the wells throughout the temperature range
  • Unrestricted access and easy-to-use software
  • Piko format design for significant savings in plate and reagent consumption
  • Remote control and monitoring over Ethernet connection or stand-alone control using USB flash drives

The PikoReal Real-Time PCR System offers users unlimited access to the PikoReal Software, which introduces the Virtual Pipetting Tool for conveniently creating and editing the plate layouts. The PikoReal software offers extensive analysis options within multiple analysis modules while still being extremely easy-to-use.

More information

For more information on the PikoReal as well as promotions, see www.thermoscientificbio.com/pikoreal/.

Analysis modesAbsolute and relative quantification, melt curve analysis, allelic discrimination, high resolution melting.
Block formats24-well, 96-well (not interchangeable)
CommunicationEthernet (up to 10 instruments can be operated from a single PC) or USB
Consumables24-well and 96-well Piko PCR Plates; for 24-well block also low profile
Dimensions(W x D x H) 300 x 230 x 310 mm
Dynamic range10 logs
Heated lid controlAutomatic temperature and pressure setting
Heated lid temperature range30 – 110°C
Max cooling rate4.5°C/sec
Max heating rate> 5°C/sec
MultiplexUp to 4 targets
Operating systemsWindows XP, Windows 7
Optics: DetectionCCD
Optics: Detection range520 – 740 nm
Optics: Excitation5 LEDs
Optics: Excitation range475 – 640 nm
Power usage200 W maximum
Sample volume10 – 50 μL (PikoReal 24), 5 – 20 μL (PikoReal 96)
Sesitivity1 copy (theoretical)
Storage ConditionAmbient
Temperature accuracy±0.2°C
Temperature range 4 – 99.9°C
Temperature uniformity ±0.3°C at 95°C, ±0.15°C at 60°C, ±0.2°C at 72°C
Weight10 kg
PikoReal block formats

PikoReal block formats

PikoReal block formats

24-well and 96-well block formats maintain industry standards for well volumes and well spacing.


  1. D. Du et al., Genome-wide identification and analysis of late embryogenesis abundant (LEA) genes in Prunus mume.Mol. Biol. Rep.  Oct 21. (2012), doi: 10.1007/s11033-012-2250-3.

  2. S.-Y., Sheng-Ying, et al., Malectin forms a complex with ribophorin I for enhanced association with misfolded glycoproteins.J. Biol. Chem. 287(45), 8080-8089. (2012).

  3. A. Jozwiak et al., Sugar availability modulates polyisoprenoid and phytosterol profiles in Arabidopsis thaliana hairy root culture. Biochim. Biophys. Acta. 1831(2), 438-447, (2013). doi: 10.1016/j.bbalip.2012.11.006

  4. I. Kavanagh, Minimizing Variation in qPCR Workflows. Genet. Eng. Biotech. N. 32.1, 22-23 (2012).