Previously added items:
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.
Four template dilutions (250 to 0.25 ng) were amplified using Cy5 labelled Molecular Beacons.
A: α Tubulin amplification curve.
B: Standard curve for ABsolute qPCR Mix data.
C: Standard curve for Competitor A data.