ABsolute qPCR SYBR Green Mixes

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or a separate ROX vial. A version with no reference dye is also available as well as a master mix optimized for capillary instruments.

Highlights

• High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes

• Absolute qPCR SYBR Green Mix (no ROX) contains 2X Master Mix and no passive reference dye
• Absolute qPCR SYBR Green ROX (500 nm) Mix contains 2X Master Mix supplemented with high ROX concentration
• Absolute qPCR SYBR Green Low ROX Mix contains 2X Master Mix supplemented with low ROX concentration
• Absolute qPCR SYBR Green Fluorescein Mix contains 2X Master Mix supplemented with fluorescein
• Absolute qPCR SYBR Green Mix plus ROX Vial contains 2X Master Mix with a separate ROX vial
• Absolute qPCR SYBR Green Capillary Mix contains 2X Master Mix optimized for capillary instruments