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DyNAmo ColorFlash Probe qPCR Kit


This qPCR Kit is a superior choice for fast, quantitative real-time analysis. Easily setup qPCR reactions, minimize errors, and track pipetting with colored reagents.
  
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Thermo Scientific DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

  • Minimized risk of pipetting errors during reaction set up
  • Especially beneficial when using white reaction vessels
  • Extremely fast protocols due to combined annealing and extension step of only 15 s
  • Specific and sensitive detection of a wide range of template concentrations
  • dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination

Applications

  • Fast qPCR
  • qPCR using sequence-specific probes
  • RT-qPCR using sequence-specific probes

Includes

  • 2X master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, optimized PCR buffer, MgCl2, dNTP mix including dUTP),
  • 40X yellow sample buffer solution with yellow dye
  • 50X ROX passive reference dye.
  
Storage Condition-20 C
HazardousNo
Storage notesNote that ROX dye is light sensitive; exposure should be minimized.
DyNAmo ColorFlash color change

Blue + yellow = green

DyNAmo ColorFlash color change

DyNAmo ColorFlash qPCR Kits contain 2X Master Mix supplemented with a blue dye. The yellow 40X sample buffer is included as a separate vial. This buffer can be mixed with the samples to provide visual aid when pipetting the samples.


Reaction setup on a white PCR plate

Assists reaction setup with clear and white plates.

Reaction setup on a white PCR plate

White reaction vessels are especially good for qPCR applications as they deliver higher signal intensities than clear vessels. However, traditional colorless reaction components are poorly visible in white wells, making reaction setup more difficult. DyNAmo ColorFlash qPCR Kits overcome this difficulty and decrease the risk of pipetting errors. In the image, the blue wells contain only the 2X master mix. The green color indicates that the sample DNA with yellow color has also been added into the reaction.