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Thermo Scientific DyNAmo Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.
DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.
When using RNA as starting material, we recommend Maxima First Strand cDNA Synthesis Kit to ensure high-quality results.
DyNAmo Flash Probe qPCR Kit produces target amplicon with shorter amplification times than 9 competitor products on both conventional and fast real-time qPCR instruments.
Thirteen probe-based qPCR kits from 10 suppliers were tested according to suppliers' recommendations. DyNAmo Flash Probe qPCR Kit delivers results faster than any other kit tested. Similar time savings are achieved with DyNAmo Flash qPCR kit for SYBR Green chemistry. bp amplicon from Salmonella typhimurium invA gene with DyNAmo Flash Probe qPCR Kit (A: combined annealing and extension 15 s, total run time 67 min) and a kit from a major supplier (B: combined annealing and extension 1 min, total run time 106 min). Reaction setup and cycling was performed as recommended by the suppliers. The results show that DyNAmo Flash Probe qPCR Kit delivers higher amplification efficiency with shorter analysis time.