Comparison of qPCR cycling times between SYBR Green qPCR kits from different suppliers. All protocols were conducted as recommended by the manufacturers and set up on the DNA Engine Opticon® 2 system (Bio-Rad Laboratories, Inc). With the new DyNAmo Flash SYBR Green qPCR Kit, the cycling time can be reduced by as much as 92 min compared to other SYBR Green kits.
Amplification of a 141 bp cDNA amplicon fron human calmodulin gene with DyNAmo Flash SYBR Green qPCR Kit (A; combined annealing and extension 15 s) and a kit from another supplier (B; annealing 30 s + extension 30 s). Reaction and cycling protocols recommended by the suppliers were followed. DyNAmo Flash SYBR Green qPCR Kit delivers efficient amplification and higher signal intensity (note different fluorescence scales in A and B).