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DyNAmo Flash SYBR Green qPCR Kit


Fast qPCR results with with high signal intensity, enhanced DNA-binding stability and processivity with excellent signal-to-noise ratio.
  
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DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Highlights

  • Extremely fast protocols: Combined annealing and extension step of only 15 s
  • Specific and sensitive detection of a wide range of template concentrations
  • Included dUTP allows the use of UNG for prevention of carry-over contamination
  • Convenient 2× master mix and optimized protocol

Applications

  • Fast qPCR
  • qPCR using SYBR Green dye
  • RT-qPCR using SYBR Green dye

Includes

  • 2X master mix (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, MgCl2, dNTP mix including dUTP)
  • 50X ROX passive reference dye.
  
Storage Condition-20 C
HazardousNo
Storage notesNote that SYBR Green and ROX dyes are light sensitive; exposure should be minimized.
Bar graph comparing minimal cycling times of various SYBR Green qPCR kits from different manufacturers. DyNAmo Flash SYBR Green qPCR Kit: 70 min; Supplier A, Kit 1, 78 min; Supplier B, 106 min; Supplier C, 111 min; Supplier D, 117 min; Supplier A, Kit 2, 162 min.

Fast real-time qPCR

Bar graph comparing minimal cycling times of various SYBR Green qPCR kits from different manufacturers. DyNAmo Flash SYBR Green qPCR Kit: 70 min; Supplier A, Kit 1, 78 min; Supplier B, 106 min; Supplier C, 111 min; Supplier D, 117 min; Supplier A, Kit 2, 162 min.

Comparison of qPCR cycling times between SYBR Green qPCR kits from different suppliers. All protocols were conducted as recommended by the manufacturers and set up on the DNA Engine Opticon® 2 system (Bio-Rad Laboratories, Inc). With the new DyNAmo Flash SYBR Green qPCR Kit, the cycling time can be reduced by as much as 92 min compared to other SYBR Green kits.


Fluorescence vs Cycle, and C(t) Cycle vs. Log Quantity are charted for both DyNAmo Flash SYBR Green qPCR Kit and a competitor product. The DyNAmo kit gives 10-fold higher fluorescence with reduced background noise at earlier cycles than the competitor over the same linear C(t) range.

Efficient amplification and high signal intensity

Fluorescence vs Cycle, and C(t) Cycle vs. Log Quantity are charted for both DyNAmo Flash SYBR Green qPCR Kit and a competitor product. The DyNAmo kit gives 10-fold higher fluorescence with reduced background noise at earlier cycles than the competitor over the same linear C(t) range.

Amplification of a 141 bp cDNA amplicon fron human calmodulin gene with DyNAmo Flash SYBR Green qPCR Kit (A; combined annealing and extension 15 s) and a kit from another supplier (B; annealing 30 s + extension 30 s). Reaction and cycling protocols recommended by the suppliers were followed. DyNAmo Flash SYBR Green qPCR Kit delivers efficient amplification and higher signal intensity (note different fluorescence scales in A and B).


Citations

  1. H. Chen et al., Upregulation of PEDF expression by PARP inhibition contributes to the decrease in hyperglycemia-induced apoptosis in HUVECs. Biochem. Biophys. Res. Commun. 369, 718–724 (2008).

  2. P. Uvarov et al., A Novel N-terminal Isoform of the Neuron-specific K-Cl Cotransporter KCC2. J. Biol. Chem. 282 (42), 30570–30576 (2007).