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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.
Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.
All Maxima Probe qPCR Master Mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water.
Amplification of human PPP1CA gene was performed on serial 10-fold dilutions of Jurkat cell total RNA (from 1 ng to 1 pg). First strand cDNA was generated with the RevertAid First Strand cDNA Synthesis Kit. cDNA was amplified with the Maxima Probe/ROX qPCR Master Mix (2X) using the TaqMan® assay specific for PPP1CA. Reactions were performed on an ABI PRISM 7000 instrument. 1 pg of total RNA was successfully detected. NTC is the non-template control.
Amplification of human PGK1 gene was performed on serial 2-fold dilutions of human genomic DNA (from 0.5 µg to 1 ng) in a replicate reactions of 25 µL. Reactions were performed on an ABI PRISM® 7000 instrument. NTC is the non-template control.
M. C. Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in Polymerase Chain Reactions. Gene. 93(1), 125-128 (1 September 1990).