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Thermo Fisher Maxima SYBR Green qPCR Master Mixes are ready-to-use solutions optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).
Amplification of the human cMyc gene was performed on serial dilutions of Jurkat cell total RNA (10 ng to 100 fg). Instruments used: A: ABI® 7500. B: Corbett RotorGene® 3000.
M. C. Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in Polymerase Chain Reactions. Gene. 93, 125-128 (1990).